WB result of Thioredoxin/TRX Recombinant Rabbit mAb
Primary antibody: Thioredoxin/TRX Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Molt-4 whole cell lysate 20 µg
Lane 4: Daudi whole cell lysate 20 µg
Lane 5: HL-60 whole cell lysate 20 µg
Lane 6: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 13 kDa
Thioredoxin/TRX Recombinant Rabbit mAb (S-1862-26)
Thioredoxin/TRX Recombinant Rabbit mAb (S-1862-26)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Thioredoxin/TRX |
| Synonyms | Thioredoxin, mitochondrial; MTRX; Mt-Trx; Thioredoxin-2; TRX2; TXN2 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q99757 |
| Clone Number | S-1862-26 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, HeLa, Molt-4, Daudi, HL-60, Raji, Neruo-2a, C2C12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250-1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Thioredoxin (TRX) is a highly conserved redox-regulatory protein that plays a crucial role in maintaining cellular redox homeostasis and modulating various cellular processes. The TRX system, which includes TRX, thioredoxin reductase (TRXR), and NADPH, acts as an essential antioxidant defense mechanism against oxidative stress by reducing oxidized proteins and neutralizing reactive oxygen species (ROS). TRX exists in multiple isoforms, such as cytosolic TRX1, mitochondrial TRX2, and spermatozoa TRX, with TRX1 being the most prominent and widely studied. It functions through its redox-active site (CGPC), which can reduce disulfide bonds in target proteins and transfer electrons to peroxiredoxins to eliminate oxidative stress. TRX also regulates gene expression, DNA synthesis, cell proliferation, and apoptosis, and has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Additionally, TRX can be secreted into the extracellular space under oxidative stress, acting as a chemotactic factor for immune cells and serving as a marker of oxidative stress.
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Western Blot
WB result of Thioredoxin/TRX Recombinant Rabbit mAb
Primary antibody: Thioredoxin/TRX Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 13 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti- Thioredoxin/TRX antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti- Thioredoxin/TRX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti- Thioredoxin/TRX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti- Thioredoxin / TRX antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
