WB result of TdT Rabbit pAb
Primary antibody: TdT Rabbit pAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 58 kDa
TdT Rabbit Polyclonal Antibody
TdT Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TdT |
| Synonyms | DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase (Terminal transferase); TDT; DNTT |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | P04053 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Jurkat, Molt-4, mouse thymus, rat thymus |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Terminal deoxynucleotidyl transferase (TdT) is a unique DNA polymerase that plays a crucial role in the immune system and DNA repair. Unlike most DNA polymerases, TdT can add nucleotides to the 3′-hydroxyl end of a DNA strand in a template-independent manner. This enzyme is primarily expressed in immature B and T lymphocytes and is essential for V(D)J recombination, a process that generates the vast diversity of antigen receptors in the adaptive immune system. By adding non-templated nucleotides (N-regions) at the junctions between V, D, and J gene segments, TdT significantly increases the diversity of T-cell receptors (TCRs) and B-cell receptors (BCRs), enhancing the immune system's ability to recognize a wide range of antigens. Additionally, TdT is involved in DNA repair, particularly in the non-homologous end joining (NHEJ) pathway, where it helps repair DNA double-strand breaks. Its ability to function without a template also makes TdT a valuable tool in biotechnology and genetic research, such as in DNA sequencing and end labeling techniques.
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Picture
Western Blot
WB result of TdT Rabbit pAb
Primary antibody: TdT Rabbit pAb at 1/1000 dilution
Lane 1: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 58 kDa
WB result of TdT Rabbit pAb
Primary antibody: TdT Rabbit pAb at 1/1000 dilution
Lane 1: rat thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 58 kDa
Immunocytochemistry
ICC shows positive staining in Jurkat cells. Anti- TdT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
