WB result of SOD2/MnSOD (acetyl K68) Rabbit pAb
Primary antibody: SOD2/MnSOD (acetyl K68) Rabbit pAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
SOD2/MnSOD (acetyl K68) Rabbit Polyclonal Antibody
SOD2/MnSOD (acetyl K68) Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SOD2/MnSOD (acetyl K68) |
| Synonyms | Superoxide dismutase [Mn], mitochondrial |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P04179 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Hu |
Background
SOD2 (acetyl K68) refers to the acetylation of lysine 68 (K68) on superoxide dismutase 2 (SOD2), a mitochondrial antioxidant enzyme critical for neutralizing superoxide radicals and protecting cells from oxidative stress. This post-translational modification, mediated by acetyltransferases such as SIRT3 (which can also deacetylate it), enhances SOD2 activity, improving its ability to scavenge reactive oxygen species (ROS) and maintain mitochondrial function. Acetylation at K68 influences SOD2’s structural stability and enzymatic efficiency, playing a key role in aging, metabolic regulation, and diseases like cancer, neurodegeneration, and cardiovascular disorders. Dysregulation of SOD2 (acetyl K68) is linked to increased oxidative damage, making it a potential therapeutic target for conditions involving mitochondrial dysfunction and oxidative stress.
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Western Blot
WB result of SOD2/MnSOD (acetyl K68) Rabbit pAb
Primary antibody: SOD2/MnSOD (acetyl K68) Rabbit pAb at 1/1000 dilution
Lane 1: rat kidney lysate 20 µg
Lane 2: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
Dot Blot
Dot blot result of SOD2/MnSOD (acetyl K68) Rabbit pAb
Lane 1: SOD2/MnSOD (acetyl K68) peptide
Lane 2: SOD2/MnSOD (acetyl K68) unmodified peptide
Primary antibody: SOD2/MnSOD (acetyl K68) Rabbit pAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell carcinoma. Anti-SOD2/MnSOD (acetyl K68) antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
