WB result of SLIT2 Rabbit mAb
Primary antibody: SLIT2 Rabbit mAb at 1/1000 dilution
Lane 1: T-47D whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Negative control: T-47D whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 170 kDa
Observed MW: 200 kDa
(This blot was developed with high sensitivity substrate)
SLIT2 Recombinant Rabbit mAb (S-730-41)
SLIT2 Recombinant Rabbit mAb (S-730-41)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Slit homolog 2 protein, Slit-2, SLIL3 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | O94813 |
| Clone Number | S-730-41 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:100 | null |
Background
Slit guidance ligand 2 (Slit-2) is a new type of adipoprotein, and the full-length Slit-2 protein is a secretory ligand. This protein splits into two fragments, a 140 kDa N-terminal product (Slit-2-N) and a 50–60 kDa C-terminal product (Slit-2-C), and interacts with Robo receptors. Robo receptors are divided into Robo1, Robo2, Robo3 and Robo4, and the binding of Slit-2 to specific Robo receptors regulates specific cell functions. Slit-2 has been reported to play an important role in neuronal and vascular development. Slit-2 is also involved in the development of many organs and is related to cancer apoptosis, migration, invasion, occurrence and development. In addition, Slit-2 can regulate different inflammatory diseases and inflammatory phenotypes and then determine the activity and severity of the disease.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SLIT2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-SLIT2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SLIT2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-SLIT2 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
