WB result of SLC40A1/Ferroportin Recombinant Rabbit mAb
Primary antibody: SLC40A1/Ferroportin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled A431 whole cell lysate 20 µg
Lane 2: unboiled Caco-2 whole cell lysate 20 µg
Lane 3: unboiled T-47D whole cell lysate 20 µg
Negative control: unboiled A431 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 60-70 kDa
SLC40A1/Ferroportin Recombinant Rabbit mAb (S-2172-35)
SLC40A1/Ferroportin Recombinant Rabbit mAb (S-2172-35)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SLC40A1/Ferroportin |
| Synonyms | Ferroportin; Ferroportin-1; Iron-regulated transporter 1; Solute carrier family 40 member 1Imported; FPN; FPN1; IREG1; SLC11A3 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9NP59 |
| Clone Number | S-2172-35 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Caco-2, T-47D, mouse spleen, mouse liver, rat spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
SLC40A1, also known as Ferroportin, is a crucial iron exporter protein that plays a vital role in iron homeostasis. It is primarily located in the plasma membrane of cells involved in iron metabolism, such as enterocytes, hepatocytes, and macrophages. In enterocytes, Ferroportin facilitates the transport of iron absorbed from the diet into the bloodstream. In hepatocytes and macrophages, it helps in the release of stored iron for systemic use. This protein contains multiple transmembrane domains and functions by moving iron ions from the intracellular space to the extracellular environment. Mutations in the SLC40A1 gene can lead to conditions like hemochromatosis, where iron overload occurs due to impaired iron export. Its regulation is also influenced by hepcidin, a hormone that binds to Ferroportin and induces its internalization and degradation, thereby controlling iron release from cells.
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Western Blot
WB result of SLC40A1/Ferroportin Recombinant Rabbit mAb
Primary antibody: SLC40A1/Ferroportin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse spleen lysate 20 µg
Lane 2: unboiled mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 65 kDa
WB result of SLC40A1/Ferroportin Recombinant Rabbit mAb
Primary antibody: SLC40A1/Ferroportin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 65 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-SLC40A1/Ferroportin antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in T47D cells (top panel) and negative staining in A431 cells (below panel). Anti- SLC40A1/Ferroportin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
