WB result of SAPK/JNK Rabbit pAb
Primary antibody: SAPK/JNK Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 39, 50 kDa
SAPK/JNK Rabbit Polyclonal Antibody
SAPK/JNK Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SAPK/JNK |
| Synonyms | Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c (SAPK1c); Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; JNK1; PRKM8; SAPK1; SAPK1C; MAPK8 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus, Synapse |
| Accession | P45983 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, NIH/3T3, mouse brain, C6, rat brain |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Ms |
Background
Stress-activated protein kinases (SAPK)/c-Jun N-terminal kinases (JNK) are members of the mitogen-activated protein kinase (MAPK) family, activated by various environmental stresses such as UV irradiation, inflammatory cytokines, and growth factors. They play crucial roles in regulating cell proliferation, apoptosis, motility, metabolism, DNA repair, and immune responses. The JNK/SAPK pathway involves a three-tiered kinase cascade, with MAPK kinases (MKKs) like MKK4 and MKK7 phosphorylating and activating JNKs. This pathway is essential for cellular adaptation to stress and has been implicated in diseases such as cancer and metabolic disorders.
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Picture
Western Blot
WB result of SAPK/JNK Rabbit pAb
Primary antibody: SAPK/JNK Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 39, 50 kDa
WB result of SAPK/JNK Rabbit pAb
Primary antibody: SAPK/JNK Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 39, 50 kDa
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Anti- SAPK/JNK antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
