WB result of S100A11 Rabbit mAb
Primary antibody: S100A11 Rabbit mAb at 1/1000 dilution
Lane 1: BxPC-3 whole cell lysate 20 µg
Lane 2: SK-OV-3 whole cell lysate 20 µg
Lane 3: T-47D whole cell lysate 20 µg
Lane 4: LoVo whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12 kDa
Observed MW: 12 kDa
S100A11 Recombinant Rabbit mAb (S-821-4)
S100A11 Recombinant Rabbit mAb (S-821-4)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Protein S100-A11, Calgizzarin, Metastatic lymph node gene 70 protein (MLN 70), Protein S100-C, S100 calcium-binding protein A11 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P31949 |
| Clone Number | S-821-4 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Rb |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:500 | |
| ICC | 1:50 |
Background
The S100A11 protein is a member of the S100 protein family, which belongs to the low-molecular-weight protein binding group of EF-hand calcium proteins and is expressed exclusively in vertebrates. S100A11 protein, also known as calgizzarin or 100C, is a small acidic protein containing two EF-hand calcium-binding motifs. This protein plays crucial roles in various biological processes. It plays a vital role in promoting keratinocyte differentiation and keratinization, participating in important processes related to skin development and integrity. It may contribute to regulating the molecular mechanisms underlying keratinocyte maturation and specialized functions. In the growth regulation of human keratinocytes, S100A11 protein can inhibit Ca2+-induced and stimulate growth by enhancing the activity of epidermal growth factor protein. S100A11 protein also plays a role in some cancers. For example, overexpression of S100A11 is associated with low survival rates in patients with pancreatic cancer, and it may affect the malignant progression of pancreatic cancer by influencing the pentose phosphate pathway. Additionally, knockdown of S100A11 can inhibit the PPP by suppressing transketolase (TKT) mRNA synthesis, providing new insights into the potential value of S100A11 in cancer therapy.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-S100A11 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-S100A11 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-S100A11 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-S100A11 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-S100A11 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in PC-3 cells. Anti- S100A11 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
