| Host | Rabbit |
| Antigen | Keratin 10 |
| Synonyms | Cytokeratin-10, CK-10, KRT10 |
| Immunogen | N/A |
| Accession | P13645 |
| Clone Number | SDT-R067 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.125 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| WB | 1:1000-1:2000 | |
| IHC-P | 1:1000 | |
| IP | 1:25 |
There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).Keratin 10 plays a role in the establishment of the epidermal barrier on plantar skin.
WB result of Keratin 10 Rabbit mAb
Primary antibody: Keratin 10 Rabbit mAb at 1/1250 dilution
Lane 1: Raw 264.7 whole cell lysate 20 µg
Lane 2: mouse skin lysate 20 µg
Negative control: RAW 264.7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
Exposure time: 7s
Keratin 10 Rabbit mAb at 1/25 dilution (0.5 µg) immunoprecipitating Keratin 10 in 0.2 mg K562 whole cell lysate.
Western blot was performed on the immunoprecipitate using Keratin 10 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: K562 whole cell lysate 5 µg (Input)
Lane 2: Keratin 10 Rabbit mAb IP in K562 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
Predicted MW: 60 kDa
Observed MW: 60 kDa
Exposure time: 90 s
IHC shows positive staining in paraffin-embedded human skin. Anti-Keratin 10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Keratin 10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skin. Anti-Keratin 10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skin. Anti-Keratin 10 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
