WB result of RNF40 Recombinant Rabbit mAb
Primary antibody: RNF40 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 130 kDa
RNF40 Recombinant Rabbit mAb (S-1068-279)
RNF40 Recombinant Rabbit mAb (S-1068-279)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RNF40 |
| Synonyms | E3 ubiquitin-protein ligase BRE1B; BRE1-B; 95 kDa retinoblastoma-associated protein (RBP95); RING finger protein 40; RING-type E3 ubiquitin transferase BRE1B; BRE1B; KIAA0661 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | O75150 |
| Clone Number | S-1068-279 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | MCF7, HeLa, HCT 116, HepG2, RAW264.7, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
RNF40 (Ring Finger Protein 40) is an E3 ubiquitin ligase that plays a critical role in histone H2B monoubiquitination at lysine 120 (H2Bub1), which is essential for gene regulation and chromatin remodeling. This protein is involved in various biological processes, including DNA damage repair, apoptosis, and cancer development. Recent studies have highlighted its dual roles in cancer progression. For example, in triple-negative breast cancer (TNBC), RNF40 supports cancer stem cell properties and glycolytic programs, promoting tumor aggressiveness. Additionally, RNF40 regulates lipid metabolism by targeting LIMA1 for proteasome-mediated degradation, thereby influencing cellular sensitivity to lipid synthesis inhibitors. These findings underscore RNF40's multifaceted roles in epigenetic regulation and cancer biology, making it a potential therapeutic target.
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Western Blot
WB result of RNF40 Recombinant Rabbit mAb
Primary antibody: RNF40 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 130 kDa
WB result of RNF40 Recombinant Rabbit mAb
Primary antibody: RNF40 Recombinant Rabbit mAb at 1/10000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 114 kDa
Observed MW: 130 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-RNF40 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
