WB result of RIBEYE/CTBP2 Rabbit pAb
Primary antibody: RIBEYE/CTBP2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SK-BR-3 whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 49 kDa
RIBEYE/CTBP2 Rabbit Polyclonal Antibody
RIBEYE/CTBP2 Rabbit Polyclonal Antibody
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RIBEYE/CTBP2 |
| Synonyms | C-terminal-binding protein 2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus, Synapse |
| Accession | P56545 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, SK-BR-3, SH-SY5Y, MCF7, mouse brain, mouse stomach, rat brain, rat stomach |
| Predicted Reactivity | Bv |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:1000 | Hu |
Background
The RIBEYE protein is a core structural component of the synaptic ribbon in retinal bipolar cells and photoreceptor cells. It is one of two isoforms (the shorter B-domain-deficient variant) encoded by the CTBP2 gene through an alternative promoter. Functioning as a scaffold protein of the synaptic ribbon, RIBEYE interacts with other synaptic components (such as RIM and Piccolo) via its unique N-terminal A-domain, while its C-terminal D-domain—homologous to CTBP1/2—mediates dimerization and may participate in metabolic regulation. This protein plays a crucial role in synaptic vesicle tethering, organizing release sites, and coordinating calcium signaling. Mutations in RIBEYE are associated with retinal disorders and hearing impairments. Research on RIBEYE provides insights into the mechanisms of specialized synaptic transmission and the pathological basis of neuro-sensory diseases.
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Western Blot
WB result of RIBEYE/CTBP2 Rabbit pAb
Primary antibody: RIBEYE/CTBP2 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 49 kDa
WB result of RIBEYE/CTBP2 Rabbit pAb
Primary antibody: RIBEYE/CTBP2 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Lane 2: rat stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 49 kDa
Observed MW: 49 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-RIBEYE/CTBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-RIBEYE/CTBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-RIBEYE/CTBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-RIBEYE/CTBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-RIBEYE/CTBP2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in SK-BR-3 cells. Anti- RIBEYE/CTBP2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
