Flow cytometric analysis of C57BL/6 mouse splenocytes labeling Rat mAb IgG2b Isotype Control at 1/20 dilution (1 μg) / (Left panel) compared with CD8α antibody at 1/200 (1 μg) dilution (S0B0498) / (Right panel). Goat Anti-Rat IgG Alexa Fluor 488 was used as the secondary antibody. Then cells were stained with CD4 - Brilliant Violet 421™ separately. Gated on total viable cells.
Rat mAb IgG2b Isotype Control (S-R486)
Rat mAb IgG2b Isotype Control (S-R486)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Rat IgG2b |
| Clone Number | S-R486 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | IHC-P, FCM |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target.
Picture
Picture
FC
Immunohistochemistry
IHC shows negative staining in paraffin-embedded mouse spleen. Rat mAb IgG2b Isotype Control was used at 1/80 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
