Flow cytometric analysis of C57BL/6 mouse splenocytes labeling Rat mAb IgG2b Isotype Control at 1/20 dilution (1 μg) / (Left panel) compared with CD8α antibody at 1/200 (1 μg) dilution (S0B0498) / (Right panel). Goat Anti-Rat IgG Alexa Fluor 488 was used as the secondary antibody. Then cells were stained with CD4 - Brilliant Violet 421™ separately. Gated on total viable cells.
Rat mAb IgG2b Isotype Control (S-R486)
Rat mAb IgG2b Isotype Control (S-R486)
Price:
Regular price
$85 USD
Regular price
Sale price
$85 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rat |
| Clone Number | S-R486 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | IHC-P, FCM |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Background
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
Picture
Picture
FC
Immunohistochemistry
IHC shows negative staining in paraffin-embedded mouse spleen. Rat mAb IgG2b Isotype Control was used at 1/80 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
