Flow cytometric analysis of C57BL/6 mouse bone marrow labeling mouse Ly-6G at 1/2000 (0.1 μg) dilution / (Right panel) compared with a Rat IgG, Isotype Control / (Left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD11b - Brilliant Violet 510 separately. Gated on myeloid cells.
Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody
Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Mouse Ly-6G/Ly-6C (Gr-1) |
| Synonyms | Lymphocyte antigen 6G, Ly-6G.1 |
| Location | Cell membrane |
| Accession | P35461 |
| Clone Number | S-R425 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:2000 |
Background
Ly-6G protein is a GPI (glycosylphosphatidylinositol)-anchored protein. Ly-6G is primarily expressed on monocytes, neutrophils, and T cells. It plays a crucial role in inhibiting anti-tumor efficacy and T-cell responses. Due to its specific expression on neutrophils, Ly-6G has been widely used as a surface marker in flow cytometry, cell sorting, and immune phenotyping experiments. Unique expression of Ly-6G in bone marrow indicates its involvement in hematopoietic microenvironment, potentially regulating the development or function of immune cells within the bone marrow niche.
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