WB result of Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb
Primary antibody: Phospho-p70 S6 Kinase (Ser371) Recombinant mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: phosphatase treated 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 55~70 kDa
Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb (S-1239-6)
Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb (S-1239-6)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-p70 S6 Kinase (Ser371) |
| Synonyms | Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1 (P70S6K1; p70-S6K 1), Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha (p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA), RPS6KB1, STK14A |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P23443 |
| Clone Number | S-1239-6 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu |
| Predicted Reactivity | Bv, Rb, Rt, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| IP | 1:50 |
Picture
Picture
Western Blot
IP
Phospho-p70 S6 Kinase (Ser371) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-p70 S6 Kinase (Ser371) in 0.4 mg 293T whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-p70 S6 Kinase (Ser371) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: 293T whole cell lysate 20 µg (Input)
Lane 2: Phospho-p70 S6 Kinase (Ser371) Rabbit mAb IP in 293T whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in 293T whole cell lysate
Predicted MW: 59 kDa
Observed MW: 55~70 kDa
Dot Blot
Dot blot result of Phospho-p70 S6 Kinase (Ser371) Recombinant mAb
Lane 1: Phospho-p70 S6 Kinase (Ser371) peptide
Lane 2: Phospho-p70 S6 Kinase unmodified peptide
Primary antibody: Phospho-p70 S6 Kinase (Ser371) Recombinant mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
