WB result of Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb
Primary antibody: Phospho-p70 S6 Kinase (Ser371) Recombinant mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: phosphatase treated 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 55~70 kDa
Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb (S-1239-6)
Phospho-p70 S6 Kinase (Ser371) Recombinant Rabbit mAb (S-1239-6)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-p70 S6 Kinase (Ser371) |
| Synonyms | Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1 (P70S6K1; p70-S6K 1), Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha (p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA), RPS6KB1, STK14A |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P23443 |
| Clone Number | S-1239-6 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu |
| Predicted Reactivity | Bv, Rb, Rt, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| IP | 1:50 |
Background
Phospho-p70 S6 Kinase (Ser371) refers to the specifically activated form of p70 ribosomal protein S6 kinase (p70S6K, a key serine/threonine kinase) phosphorylated at its serine 371 residue (Ser371). As a central downstream effector of the mTORC1 (mechanistic target of rapamycin complex 1) signaling pathway, phosphorylation at this site is crucial for the full activation of p70S6K. Ser371 is located within the activation loop (T-loop) of the kinase domain. Its phosphorylation, typically catalyzed by mTORC1 itself or other upstream kinases, significantly enhances the kinase activity of p70S6K. The activated Phospho-p70 S6 Kinase (Ser371) then phosphorylates its downstream targets, such as ribosomal protein S6 and eIF4B, thereby directly promoting protein translation, ribosome biogenesis, and cell growth. Consequently, detecting the phosphorylation level at Ser371 is a commonly used biomarker for assessing mTORC1 pathway activity and studying cell proliferation, metabolic regulation, as well as drug target responses in related diseases such as cancer and metabolic disorders.
Picture
Picture
Western Blot
IP
Phospho-p70 S6 Kinase (Ser371) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-p70 S6 Kinase (Ser371) in 0.4 mg 293T whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-p70 S6 Kinase (Ser371) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: 293T whole cell lysate 20 µg (Input)
Lane 2: Phospho-p70 S6 Kinase (Ser371) Rabbit mAb IP in 293T whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in 293T whole cell lysate
Predicted MW: 59 kDa
Observed MW: 55~70 kDa
Dot Blot
Dot blot result of Phospho-p70 S6 Kinase (Ser371) Recombinant mAb
Lane 1: Phospho-p70 S6 Kinase (Ser371) peptide
Lane 2: Phospho-p70 S6 Kinase unmodified peptide
Primary antibody: Phospho-p70 S6 Kinase (Ser371) Recombinant mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
