WB result of Phospho-p38 MAPK (Thr180) Recombinant Rabbit mAb
Primary antibody: Phospho-p38 MAPK (Thr180) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 25 μM Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38 kDa
Phospho-p38 MAPK (Thr180) Recombinant Rabbit mAb (S-1553-9)
Phospho-p38 MAPK (Thr180) Recombinant Rabbit mAb (S-1553-9)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-p38 MAPK (Thr180) |
| Synonyms | Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein (CSAID-binding protein; CSBP); MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha (MAP kinase p38 alpha); Stress-activated protein kinase 2a (SAPK2a); CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; MAPK14 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q16539 |
| Clone Number | S-1553-9 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Dg, Cp, Cz, Zf |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Phospho-p38 MAPK (Thr180) is the dual-phosphorylated, enzymatically active form of the p38 mitogen-activated protein kinase in which the conserved threonine residue at position 180 (within the canonical Thr-X-Tyr activation motif, TGY in human p38α) is phosphorylated, together with Tyr182, by upstream MAP kinase kinases (MKK3/6 or MKK4), resulting in a conformational switch that opens the ATP- and substrate-binding sites, thereby enabling the kinase to transduce stress, inflammatory, and cytokine signals to downstream transcription factors (e.g., ATF-2, CHOP, MEF2C) and other effectors, ultimately governing cellular responses such as apoptosis, differentiation, and cytokine production.
Picture
Picture
Western Blot
WB result of Phospho-p38 MAPK (Thr180) Recombinant Rabbit mAb
Primary antibody: Phospho-p38 MAPK (Thr180) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 38 kDa
Dot Blot
Dot blot result of Phospho-p38 MAPK (Thr180) Recombinant Rabbit mAb
Lane 1: p38 MAPK (Thr180) phospho peptide 1
Lane 2: p38 MAPK (Thr180) phospho peptide 2
Lane 3: p38 MAPK (Thr182) phospho peptide
Lane 4: p38 MAPK unmodified peptide
Primary antibody: Phospho-p38 MAPK (Thr180) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
