WB result of Phospho-FAK (Tyr397) Rabbit pAb
Primary antibody: Phospho-FAK (Tyr397) Rabbit pAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 10 mM Pervanadate for 60 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 119 kDa
Observed MW: 120 kDa
Phospho-FAK (Tyr397) Rabbit Polyclonal Antibody
Phospho-FAK (Tyr397) Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-FAK (Tyr397) |
| Synonyms | Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase (FRNK); Protein phosphatase 1 regulatory subunit 71 (PPP1R71); Protein-tyrosine kinase 2; p125FAK; pp125FAK; FAK; FAK1; PTK2 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Cytoplasm, Cytoskeleton, Nucleus |
| Accession | Q05397 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms |
| Predicted Reactivity | Ck, Rt, Xe |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu |
Background
Phospho-FAK (Tyr397) is a crucial phosphorylated form of the focal adhesion kinase (FAK) protein, which plays a significant role in various cellular processes. FAK is a non-receptor protein tyrosine kinase that is involved in the regulation of cell adhesion, migration, and survival. When phosphorylated at tyrosine residue 397, FAK becomes activated and serves as a binding site for other signaling molecules, such as Src, thereby initiating downstream signaling pathways that are essential for cell motility and the formation of focal adhesions. Dysregulation of Phospho-FAK (Tyr397) has been implicated in several diseases, including cancer, where it contributes to tumor progression and metastasis by enhancing cell invasion and survival.
Picture
Picture
Western Blot
WB result of Phospho-FAK (Tyr397) Rabbit pAb
Primary antibody: Phospho-FAK (Tyr397) Rabbit pAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 10 mM Pervanadate for 60 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 119 kDa
Observed MW: 120 kDa
Immunocytochemistry
ICC analysis of HeLa cells treated with Pervanadate (10mM, 60min) (top panel) and untreated HeLa cells (below panel). Anti- Phospho-FAK (Tyr397) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
