WB result of Phospho-Akt (Ser473) Rabbit mAb
Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
Phospho-Akt (Ser473) Recombinant Rabbit mAb (S-510-64)
Phospho-Akt (Ser473) Recombinant Rabbit mAb (S-510-64)
Price:
Regular price
$130.00 SGD
Regular price
Sale price
$130.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Akt (Ser473) |
| Synonyms | RAC-alpha serine/threonine-protein kinase, Protein kinase B (PKB), Protein kinase B alpha (PKB alpha), Proto-oncogene c-Akt, RAC-PK-alpha, PKB, RAC, AKT1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P31749、Q9Y243、P31751 |
| Clone Number | S-510-64 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP, Dot blot |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| Dot bolt | 1:1000 | |
| IP | 1:50 |
Background
Akt or Protein kinase B, is a serine/threonine kinase that plays an important role in regulating a number of cellular processes such as growth, metabolism and survival. The importance of the Akt pathway is highlighted by the mutation of various components of the pathway in human cancers such as the PTEN and PI3-kinase (P110α), which occur in more than 30% of human tumors. For Akt to achieve full activation, phosphorylation is needed at both serine 473 (ser473) of the hydrophobic tail and threonine 308 (thr308) of the activation motif, upon growth factor ligation to the receptor tyrosine kinases.
Picture
Picture
Western Blot
WB result of Phospho-Akt (Ser473) Rabbit mAb
Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
WB result of Phospho-Akt (Ser473) Rabbit mAb
Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: C6 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 60 kDa
IP
Phospho-Akt (Ser473) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Phospho-Akt (Ser473) in 0.4 mg NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate.
Western blot was performed on the immunoprecipitate using Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate 20 µg (Input)
Lane 2: Phospho-Akt (Ser473) Rabbit mAb IP in NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in NIH/3T3 treated with calyculin A (100 nM, 30 min) whole cell lysate
Predicted MW: 56 kDa
Observed MW: 60 kDa
Dot Blot
Dot blot result of Phospho-Akt (Ser473) Rabbit mAb
Lane 1: phospho-Akt (Ser473) peptide
Lane 2: Akt peptide
Primary antibody: Phospho-Akt (Ser473) Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
