ICC shows positive staining in HeLa cells. Anti- Pericentrin antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (magenta).
Pericentrin Recombinant Mouse mAb (S-R144)
Pericentrin Recombinant Mouse mAb (S-R144)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Pericentrin |
| Synonyms | Kendrin, Pericentrin-B, PCNT, |
| Accession | O95613 |
| Clone Number | S-R144 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | ICC |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:50 | null |
Background
Pericentrin (kendrin), also known as PCNT and pericentrin-B (PCNTB), is a protein which in humans is encoded by the PCNT gene on chromosome 21 [PMID: 8812505; PMID 10823944; PMID 11171385]. This protein localizes to the centrosome and recruits proteins to the pericentriolar matrix (PCM) to ensure proper centrosome and mitotic spindle formation, and thus, uninterrupted cell cycle progression [PMID: 20567258; PMID: 24466316; PMID: 21270239; PMID: 18955030]. This gene is implicated in many diseases and disorders.
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Immunocytochemistry
