Flow cytometric analysis of Human CD14 expression on human peripheral blood cells. Human peripheral blood cells were stained with PE Rabbit IgG Isotype Control (Left panel) or SDT PE Rabbit Anti-Human CD14 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
PE Rabbit Anti-Human CD14 Antibody (S-395-8)
PE Rabbit Anti-Human CD14 Antibody (S-395-8)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD14 |
| Synonyms | Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cell membrane |
| Accession | P08571 |
| Clone Number | S-395-8 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human peripheral blood cells |
| Purification | Protein A |
| Concentration | 0.02mg/ml |
| Conjugation | PE |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein primarily expressed on monocytes, macrophages, and some granulocytes. It functions as a pattern recognition receptor (PRR) and plays a crucial role in the innate immune response by recognizing bacterial lipopolysaccharides (LPS), pathogen-associated molecular patterns (PAMPs), and damage-associated molecular patterns (DAMPs). CD14 exists in two forms: membrane-bound CD14 (mCD14) and soluble CD14 (sCD14), which can be secreted by activated cells. It acts as a co-receptor for Toll-like receptor 4 (TLR4) and enhances the immune response to LPS by facilitating its delivery to the TLR4-MD2 complex. Additionally, CD14 is involved in TLR4-independent signaling pathways and contributes to tissue homeostasis by promoting the clearance of apoptotic cells.
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