WB result of O-Linked N-Acetylglucosamine Rabbit mAb
Primary antibody: O-Linked N-Acetylglucosamine Rabbit mAb at 1/500 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple kDa
Observed MW: Multiple kDa
(This blot was developed with high sensitivity substrate)
O-Linked N-Acetylglucosamine Recombinant Rabbit mAb (S-R256)
O-Linked N-Acetylglucosamine Recombinant Rabbit mAb (S-R256)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | O-Linked N-Acetylglucosamine |
| Synonyms | O-GlcNAc |
| Clone Number | S-R256 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | |
| IHC | 1:500 | |
| ICC | 1:500 |
Background
O-GlcNAc (short for O-linked GlcNAc or O-linked β-N-acetylglucosamine) is a reversible enzymatic post-translational modification that is found on serine and threonine residues of nucleocytoplasmic proteins. The modification is characterized by a β-glycosidic bond between the hydroxyl group of serine or threonine side chains and N-acetylglucosamine (GlcNAc). It has been suggested that apoptosis is regulated by O-GlcNAc. In various cancers, elevated O-GlcNAc levels have been reported to suppress apoptosis. Caspase-3, caspase-8, and caspase-9 have been reported to be modified by O-GlcNAc. Caspase-8 is modified near its cleavage/activation sites; O-GlcNAc modification may block caspase-8 cleavage and activation by steric hindrance. Pharmacological lowering of O-GlcNAc with 5S-GlcNAc accelerated caspase activation while pharmacological raising of O-GlcNAc with thiamet-G inhibited caspase activation.
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Western Blot
WB result of O-Linked N-Acetylglucosamine Rabbit mAb
Primary antibody: O-Linked N-Acetylglucosamine Rabbit mAb at 1/500 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple kDa
Observed MW: Multiple kDa
(This blot was developed with high sensitivity substrate)
WB result of O-Linked N-Acetylglucosamine Rabbit mAb
Primary antibody: O-Linked N-Acetylglucosamine Rabbit mAb at 1/500 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple kDa
Observed MW: Multiple kDa
(This blot was developed with high sensitivity substrate)
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-O-Linked N-Acetylglucosamine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-O-Linked N-Acetylglucosamine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-O-Linked N-Acetylglucosamine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-O-Linked N-Acetylglucosamine antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-O-Linked N-Acetylglucosamine antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
