WB result of NF-kB p65 (acetyl K310) Recombinant Rabbit mAb
Primary antibody: NF-kB p65 (acetyl K310) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T transfected with NF-kB p65 -myc-His fusion protein whole cell lysate 20 µg
Lane 2: 293T transfected with NF-kB p65-myc-His fusion protein and P300 (aa1287-1663)-myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 70 kDa
NF-kB p65 (acetyl K310) Recombinant Rabbit mAb (S-1113-161)
NF-kB p65 (acetyl K310) Recombinant Rabbit mAb (S-1113-161)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NF-kB p65 (acetyl K310) |
| Synonyms | Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; NFKB3; RELA |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q04206 |
| Clone Number | S-1113-161 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
NF-kB p65 (acetyl K310) is the acetylated form of the p65 (RelA) subunit of the NF-κB transcription factor complex, wherein lysine 310 is acetylated primarily by the histone acetyltransferases CBP/p300, a modification that boosts its transcriptional activity without enhancing DNA binding or weakening IκB association; this acetylation, often preceded by phosphorylation at Ser 536 or Ser 276, recruits the bromodomain protein BRD4 to amplify expression of NF-κB-dependent inflammatory and immune genes, is reversed by HDAC3 and SIRT2 deacetylases to terminate signaling, and is dynamically regulated in physiology and diseases such as cancer, chronic inflammation and infection.
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Western Blot
