Flow cytometric analysis of C57BL/6 mouse bone marrow labeling Mouse TER-119/Erythroid Cells at 1/2000 (0.1 μg) dilution / (Right panel) compared with a Rat IgG, Isotype Control / (Left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD45 - PE/Cy7 separately. Gated on total viable cells.
Mouse TER-119/Erythroid Cells Recombinant Rat mAb (S-R517)
Mouse TER-119/Erythroid Cells Recombinant Rat mAb (S-R517)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | TER-119/Erythroid cells |
| Synonyms | Lymphocyte antigen 76; Ly76; Ly-76; Ter119 |
| Location | Cell membrane |
| Accession | 104231 |
| Clone Number | S-R517 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | IHC-P, FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:500 | Ms |
| FCM | 1:2000 | Ms |
Background
TER-119 is a monoclonal antibody that specifically binds to the Ly76 protein associated with erythroid cells in mice. Ly76 is a glycophorin A-associated protein that is expressed from early proerythroblasts to mature erythrocytes, but not on BFU-E or CFU-E. The TER-119 antibody is commonly used in flow cytometry to identify and exclude red blood cells, allowing for more accurate analysis of other blood cell types, such as in immune cell typing or hematopoietic progenitor studies. In research, the TER-119 antibody helps to distinguish between different stages of red blood cell development. For example, by combining TER-119 with other cell surface molecular markers such as CD71 and CD44, erythroid cells in mouse bone marrow can be differentiated into five stages, including proerythroblasts, basophilic erythroblasts, polychromatophilic erythroblasts, orthochromatic erythroblasts, and mature red blood cells. Additionally, the TER-119 antibody is used to study regulatory mechanisms in the process of erythropoiesis, such as the regulation of CD71+TER119+ erythroid progenitor cells by CD45.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Mouse TER-119/Erythroid Cells antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Mouse TER-119/Erythroid Cells antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Mouse TER-119/Erythroid Cells antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
