WB result of M6PR Recombinant Rabbit mAb
Primary antibody: M6PR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: SK-MEL-28 whole cell lysate 20 µg
Low expression control: A549 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 274 kDa
Observed MW: 310 kDa
M6PR Recombinant Rabbit mAb (S-2348-14)
M6PR Recombinant Rabbit mAb (S-2348-14)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | M6PR |
| Synonyms | Cation-independent mannose-6-phosphate receptor; CI Man-6-P receptor; CI-MPR; 300 kDa mannose 6-phosphate receptor (MPR 300); Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor (IGF-II receptor); M6P/IGF2 receptor (M6P/IGF2R); CD222; MPRI; IGF2R |
| Immunogen | Synthetic Peptide |
| Location | Golgi apparatus membrane |
| Accession | P11717 |
| Clone Number | S-2348-14 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | 293T, HeLa, Jurkat, SK-MEL-28, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu |
| IF | 1:100 | Hu |
Background
M6PR，which stands for Mannose-6-Phosphate Receptor，is a crucial protein in the cellular processes of eukaryotic organisms. It primarily resides in the Golgi apparatus and endosomes. The M6PR has the ability to recognize and bind to mannose-6-phosphate residues that are present on certain lysosomal enzymes. This binding is essential for the proper sorting and transport of these enzymes. When lysosomal enzymes are synthesized in the endoplasmic reticulum and then transported to the Golgi apparatus，they are tagged with mannose-6-phosphate. The M6PR captures these tagged enzymes in the trans-Golgi network and helps to package them into clathrin-coated vesicles. These vesicles then transport the enzymes to the endosomes，where the M6PR releases the enzymes. The enzymes are subsequently delivered to the lysosomes to carry out their functions in breaking down various biomolecules. In addition to its role in intracellular transport，the M6PR also participates in the endocytosis of extracellular molecules that have been modified with mannose-6-phosphate，contributing to the cell's nutrient uptake and waste processing.
Picture
Picture
Western Blot
WB result of M6PR Recombinant Rabbit mAb
Primary antibody: M6PR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 274 kDa
Observed MW: 310 kDa
WB result of M6PR Recombinant Rabbit mAb
Primary antibody: M6PR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 274 kDa
Observed MW: 310 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-M6PR antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human testis. Anti-M6PR antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
