WB result of Ly6G Rabbit pAb
Primary antibody: Ly6G Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse bone marrow lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 25-35 kDa
Ly6G Rabbit Polyclonal Antibody
Ly6G Rabbit Polyclonal Antibody
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Ly6G |
| Synonyms | Lymphocyte antigen 6G; Ly-6G; Ly-6G.1; Ly6g |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P35461 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms |
| Positive Sample | mouse bone marrow |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein that belongs to the Ly-6 superfamily and is expressed in a developmentally regulated manner by myeloid cells, including neutrophils, granulocytes, and some monocytes. It serves as a key marker for mature neutrophils and is widely used in research to identify and deplete these cells in vivo. Ly6G is transiently expressed on monocytes during bone marrow development, while its expression on granulocytes and peripheral neutrophils correlates with their differentiation and maturation levels. This antigen plays important roles in immune responses, such as antitumor immunity and neutrophil-mediated inflammation.
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Western Blot
