WB result of Jagged1 Recombinant Rabbit mAb
Primary antibody: Jagged1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Molt-4 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: OVCAR-3 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 134 kDa
Observed MW: 130-200 kDa
Jagged1 Recombinant Rabbit mAb (S-3296)
Jagged1 Recombinant Rabbit mAb (S-3296)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Jagged1 |
| Synonyms | Protein jagged-1; hJ1; CD339; JAGL1; JAG1 |
| Location | Cell membrane |
| Accession | P78504 |
| Clone Number | S-3296 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | HT-29, OVCAR-3, HepG2 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:1000 | Hu |
Background
Jagged1 is a single-pass transmembrane ligand for the Notch signaling pathway, encoded by the JAG1 gene on chromosome 20p12.2, characterized by an extracellular region containing multiple EGF-like repeats and a DSL (Delta/Serrate/Lag-2) motif that binds Notch receptors, thereby activating transcriptional programs essential for embryogenesis, vascular development, hematopoiesis, and tissue homeostasis; mutations in JAG1 underlie Alagille syndrome, while dysregulated Jagged1-Notch signaling promotes tumor progression, cancer stem cell maintenance, and immune evasion in diverse malignancies.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Jagged1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Jagged1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Jagged1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
