Jagged1 Recombinant Rabbit mAb (S-3296)

Catalog Number: S0B6227 Application: WB, IHC-P, ICC, ICFCM Reactivity: Hu, Ms, Mk Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Jagged1
Synonyms	Protein jagged-1; hJ1; CD339; JAGL1; JAG1
Location	Cell membrane
Accession	P78504
Clone Number	S-3296
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P, ICC, ICFCM
Reactivity	Hu, Ms, Mk
Positive Sample	HT-29, OVCAR-3, HepG2
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:1000	Hu
ICC	1:500	Hu
ICFCM	1:500	Hu

Background

Jagged1 is a single-pass transmembrane ligand for the Notch signaling pathway, encoded by the JAG1 gene on chromosome 20p12.2, characterized by an extracellular region containing multiple EGF-like repeats and a DSL (Delta/Serrate/Lag-2) motif that binds Notch receptors, thereby activating transcriptional programs essential for embryogenesis, vascular development, hematopoiesis, and tissue homeostasis; mutations in JAG1 underlie Alagille syndrome, while dysregulated Jagged1-Notch signaling promotes tumor progression, cancer stem cell maintenance, and immune evasion in diverse malignancies.

Picture

Western Blot

WB result of Jagged1 Recombinant Rabbit mAb
Primary antibody: Jagged1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Molt-4 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: OVCAR-3 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 134 kDa
Observed MW: 130-200 kDa

FC

Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) labelling Jagged1 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: Jurkat

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-Jagged1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-Jagged1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Jagged1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HepG2 cells. Anti- Jagged1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).