WB result of Human IgG1 F(c) mouse mAb Primary antibody: Human IgG1 F(c) mouse mAb at 1/1000 dilution Lane 1: Human serum lysate 5 µg Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55 kDa Observed MW: 55 kDa
Human IgG1 F(c) Mouse mAb (S-448-1)
Human IgG1 F(c) Mouse mAb (S-448-1)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Human IgG1 F(c) |
| Synonyms | Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1 |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cell membrane |
| Accession | P01857 |
| Clone Number | S-448-1 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | ELISA, WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC | 1:5000 |
Background
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4 [PMID: 32370812].
Picture
Picture
Western Blot
WB result of Human IgG1 F(c) mouse mAb Primary antibody: Human IgG1 F(c) mouse mAb at 1/1000 dilution Lane 1: IgG recombinant protein lysate 20 ng Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55 kDa Observed MW: 55 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG1 F(c) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG1 F(c) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Human IgG1 F(c) antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
