WB result of Histone H3 (acetyl K36) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K36) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
Observed MW: 17 kDa
Histone H3 (acetyl K36) Recombinant Rabbit mAb (S-1732-53)
Histone H3 (acetyl K36) Recombinant Rabbit mAb (S-1732-53)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H3 (acetyl K36) |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-1732-53 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, NIH/3T3 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
Background
Histone H3 acetylation at lysine 36 (H3K36ac) is a post-translational modification that plays a crucial role in chromatin regulation. It is predominantly found at the promoters of actively transcribed genes and is associated with the regulation of transcription, as it can influence chromatin structure, promote the recruitment of transcriptional activators, and modulate the transition between active and repressive chromatin states. This modification is also implicated in processes such as splicing, nucleosome turnover, DNA repair, and the establishment of DNA methylation patterns, highlighting its importance in maintaining genomic stability and regulating gene expression.
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Western Blot
WB result of Histone H3 (acetyl K36) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K36) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
Observed MW: 17 kDa
Dot Blot
Dot blot result of Histone H3 (acetyl K36) Recombinant Rabbit mAb
Lane 1: Histone H3K36 acetyl peptide
Lane 2: Histone H3K36 unmodified peptide
Primary antibody: Histone H3 (acetyl K36) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
