Histone H3 (acetyl K36) Recombinant Rabbit mAb (S-1732-53)

Catalog Number: S0B1224 Application: WB, ChIP Reactivity: Hu, Ms Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Histone H3 (acetyl K36)
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	P68431
Clone Number	S-1732-53
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, ChIP
Reactivity	Hu, Ms
Positive Sample	HeLa, NIH/3T3
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
Dot Blot	1:1000
WB	1:1000	Hu, Ms
ChIP	1:20-1:50	Hu

Background

Histone H3 acetylation at lysine 36 (H3K36ac) is a post-translational modification that plays a crucial role in chromatin regulation. It is predominantly found at the promoters of actively transcribed genes and is associated with the regulation of transcription, as it can influence chromatin structure, promote the recruitment of transcriptional activators, and modulate the transition between active and repressive chromatin states. This modification is also implicated in processes such as splicing, nucleosome turnover, DNA repair, and the establishment of DNA methylation patterns, highlighting its importance in maintaining genomic stability and regulating gene expression.

Picture

Western Blot

WB result of Histone H3 (acetyl K36) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K36) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
Observed MW: 17 kDa

WB result of Histone H3 (acetyl K36) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K36) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
Observed MW: 17 kDa

Dot Blot

Dot blot result of Histone H3 (acetyl K36) Recombinant Rabbit mAb
Lane 1: Histone H3K36 acetyl peptide
Lane 2: Histone H3K36 unmodified peptide
Primary antibody: Histone H3 (acetyl K36) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

ChIP

Chromatin immunoprecipitation (ChIP) was performed on HeLa+TSA(500ng/ml,4h) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (acetyl K36) Recombinant Rabbit mAb (S-1732-53) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation. Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA)
showed the enrichment of RPL30, GAPDH, MYOD1,
AFM, SAT-α and SAT-2 in Histone H3 (acetyl K36) Recombinant Rabbit mAb
(S-1732-53)-immunoprecipitated sample.