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FITC Mouse Anti-Rat CD45RA Antibody (S-R629)

FITC Mouse Anti-Rat CD45RA Antibody (S-R629)

Catalog Number: S0B5011 Application: FCM Reactivity: Rat Conjugation: FITC Brand: Starter
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Regular price $143.00 SGD
Regular price Sale price $143.00 SGD
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Product Details

Product Specification


Host Mouse
Antigen Rat CD45RA
Synonyms Leukocyte common antigen; high molecular weight form
Location Cell membrane
Accession 24699
Clone Number S-R629
Antibody Type Mouse mAb
Isotype IgG1,k
Application FCM
Reactivity Rt
Positive Sample SD Rat splenocytes
Purification Protein G
Concentration 0.2 mg/ml
Conjugation FITC
Physical Appearance Liquid
Storage Buffer

PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

Dilution


application dilution species
FCM 1.25μl per million cells in 100μl volume Rt

Background

CD45RA protein is a specific isoform of the CD45 receptor, also known as protein tyrosine phosphatase receptor type C (PTPRC), which is generated through alternative splicing of the CD45 gene. It is a marker for naive T cells and central memory T cells (TCM), characterized by the presence of exon 4 (A) but the absence of exons 5 (B) and 6 (C) in its mRNA. CD45RA is expressed on the surface of nucleated hematopoietic cells, including B cells, NK cells, naive/resting T cells, medullary thymocytes, and plasmacytoid dendritic cells. It plays a crucial role in the immune system by modulating T cell receptor (TCR) and B cell receptor (BCR) signaling pathways, facilitating the activation and proliferation of immune cells. In adults, CD45RA+ cells may help prevent autoimmune diseases, while reduced numbers of CD45RA+ CD4+ T cells have been associated with conditions such as biliary atresia, primary biliary cirrhosis, fetal distress, and preeclampsia. Pathologists use CD45RA to differentiate naive T cells (CD45RA+) from memory T cells (CD45RO+), although this distinction may not be applicable in adult allogeneic hematopoietic cell transplant recipients.

Picture

FC

Flow cytometric analysis of Rat CD45RA expression on SD Rat splenocytes. SD Rat splenocytes were stained with Brilliant Violet 605™ Mouse Anti-Rat CD3 Antibody and either FITC Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Rat CD45RA Antibody (Right panel) at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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