Flow cytometric analysis of Rat CD45RA expression on SD Rat splenocytes. SD Rat splenocytes were stained with Brilliant Violet 605™ Mouse Anti-Rat CD3 Antibody and either FITC Mouse IgG1, κ Isotype Control (Left panel) or SDT FITC Mouse Anti-Rat CD45RA Antibody (Right panel) at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Product Details
Product Details
Product Specification
Host | Mouse |
Antigen | Rat CD45RA |
Synonyms | Leukocyte common antigen; high molecular weight form |
Location | Cell membrane |
Accession | 24699 |
Clone Number | S-R629 |
Antibody Type | Mouse mAb |
Isotype | IgG1,k |
Application | FCM |
Reactivity | Rt |
Positive Sample | SD Rat splenocytes |
Purification | Protein G |
Concentration | 0.2 mg/ml |
Conjugation | FITC |
Physical Appearance | Liquid |
Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
application | dilution | species |
FCM | 1.25μl per million cells in 100μl volume | Rt |
Background
CD45RA protein is a specific isoform of the CD45 receptor, also known as protein tyrosine phosphatase receptor type C (PTPRC), which is generated through alternative splicing of the CD45 gene. It is a marker for naive T cells and central memory T cells (TCM), characterized by the presence of exon 4 (A) but the absence of exons 5 (B) and 6 (C) in its mRNA. CD45RA is expressed on the surface of nucleated hematopoietic cells, including B cells, NK cells, naive/resting T cells, medullary thymocytes, and plasmacytoid dendritic cells. It plays a crucial role in the immune system by modulating T cell receptor (TCR) and B cell receptor (BCR) signaling pathways, facilitating the activation and proliferation of immune cells. In adults, CD45RA+ cells may help prevent autoimmune diseases, while reduced numbers of CD45RA+ CD4+ T cells have been associated with conditions such as biliary atresia, primary biliary cirrhosis, fetal distress, and preeclampsia. Pathologists use CD45RA to differentiate naive T cells (CD45RA+) from memory T cells (CD45RO+), although this distinction may not be applicable in adult allogeneic hematopoietic cell transplant recipients.
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