Flow cytometric analysis of Mouse H-2Kd expression on BALB/c mouse splenocytes. BALB/c mouse splenocytes were stained with FITC Mouse IgG2a, κ Isotype Control (Black line histogram) and SDT FITC Mouse Anti-Mouse H-2Kd Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Mouse H-2Kd Antibody (S-R647)
FITC Mouse Anti-Mouse H-2Kd Antibody (S-R647)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Mouse H-2Kd |
| Synonyms | H-2 class I histocompatibility antigen, K-D alpha chain; H-2K(D); H2-K1; H2-K |
| Location | Membrane |
| Accession | P01902 |
| Clone Number | S-R647 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
H-2Kd protein is a major histocompatibility complex (MHC) class I molecule expressed in mice, particularly in BALB/c strains. It plays a critical role in antigen presentation by binding to antigenic peptides derived from pathogens, such as influenza nucleoprotein, and presenting them to CD8+ T cells to initiate an immune response. The crystal structure of H-2Kd in complex with beta-2 microglobulin and an influenza-derived peptide has been elucidated, revealing specific binding motifs and the structural basis for peptide recognition. This understanding is crucial for studies on immune responses and vaccine development.
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