Flow cytometric analysis of Mouse CD45.1 expression on mouse splenocytes. Mouse splenocytes from a C57BL/6 mouse (Left Panel) and SJL mouse (Right Panel) were separately stained with FITC Mouse IgG2a, κ Isotype Control (Black line histogram) and SDT FITC Mouse Anti-Mouse CD45.1 Antibody (Red line histogram) at 5μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Mouse Anti-Mouse CD45.1 Antibody (S-R521)
FITC Mouse Anti-Mouse CD45.1 Antibody (S-R521)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Mouse CD45.1 |
| Synonyms | Ly5.1 |
| Location | Cell membrane |
| Accession | P06800 |
| Clone Number | S-R521 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | SJL mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
CD45.1 is a protein isoform of CD45, also known as PTPRC, which is a receptor-type tyrosine phosphatase expressed on all hematopoietic cells except erythrocytes and platelets. This type I transmembrane protein plays a crucial role in the activation processes of B and T cell receptors, as well as in thymic selection. CD45.1, along with CD45.2, is commonly used in adoptive cell transfer experiments to distinguish donor cells from host cells due to their allelic differences. Mice carrying the CD45.1 allele, such as C57BL/6J, are widely used in research for this purpose. CD45.1 is also used in studies to understand the rejuvenating effects of young blood on aged tissues. In heterochronic parabiosis models, where the circulatory systems of old and young mice are connected, CD45.1 and CD45.2 genotypes are used to track the cells of old and young mice, respectively.
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