Human PBMC (human peripheral blood mononuclear cells) were stained with Alexa Fluor® 647 Mouse Anti-Human CD4 antibody and fixed and permeabilized with the eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent. The cells were then stained with either FITC Armenian hamster IgG Isotype Control (Left panel) or SDT FITC Armenian hamster Anti-Mouse/Human Helios Antibody (Right panel) at 5μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
FITC Armenian hamster Anti-Mouse/Human Helios Antibody (S-R640)
FITC Armenian hamster Anti-Mouse/Human Helios Antibody (S-R640)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | Helios |
| Synonyms | Zinc finger protein Helios; Ikaros family zinc finger protein 2; IKZF2; ZNFN1A2 |
| Location | Nucleus |
| Accession | Q9UKS7、 P81183 |
| Clone Number | S-R640 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICFCM |
| Reactivity | Hu, Ms |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Hu, Ms |
Background
Helios protein (encoded by the IKZF2 gene) is a member of the Ikaros family of transcription factors and contains multiple zinc finger domains that facilitate DNA binding and the formation of homodimers or heterodimers. It is predominantly expressed in T cells, regulatory T cells (Tregs), certain B cell subsets, and hematopoietic and epithelial tissues. Helios plays a crucial role in T cell activation, proliferation, and the stability and function of Tregs. Additionally, Helios has been identified as a potential biomarker in autoimmune diseases and may influence tumor development by regulating gene expression and modulating cell differentiation and proliferation.
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