Flow cytometric analysis of TCR β expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with SDT FITC Armenian Hamster Anti-Mouse TCR β Antibody at 0.25 μg/test and either Alexa Fluor® 647 Rat Anti-Mouse CD8a Antibody (Left panel) or Brilliant Violet 421™ Rat Anti-Mouse CD4 Antibody (Right panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Product Details
Product Details
Product Specification
Host | Armenian hamster |
Antigen | Mouse TCR β |
Synonyms | T-cell receptor beta-1 chain C region |
Location | Membrane |
Accession | P01852 |
Clone Number | S-R616 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | FCM |
Reactivity | Ms |
Positive Sample | C57BL/6 mouse splenocytes |
Purification | Protein G |
Concentration | 0.05mg/ml |
Conjugation | FITC |
Physical Appearance | Liquid |
Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
application | dilution | species |
FCM | 5 μl per million cells in 100μl volume | Ms |
Background
The TCR β (T-cell receptor beta chain) is a crucial component of the T-cell receptor, which, together with the TCR α chain, forms the αβTCR on the surface of T cells. This receptor is responsible for recognizing and binding to antigenic peptide complexes presented by MHC molecules. The diversity of the TCR β chain is generated through a combination of random recombination of multiple germline gene segments, gene rearrangement, junctional diversity, N-region nucleotide insertions, and random pairing of α and β chains. These mechanisms collectively provide the TCR repertoire with immense diversity, enabling T cells to recognize a wide range of antigens.
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