Flow cytometric analysis of SW620 (Human colorectal adenocarcinoma epithelial cell, left) / KATO III (human gastric carcinoma, right) cells labelling FGFR2 antibody at 1/500 dilution (0.1 μg) / (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: SW620
FGFR2 Recombinant Rabbit mAb (S-R239)
FGFR2 Recombinant Rabbit mAb (S-R239)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FGFR2 |
| Synonyms | Fibroblast growth factor receptor 2, K-sam (KGFR), Keratinocyte growth factor receptor, CD332, BEK, KSAM |
| Location | Cell membrane |
| Accession | P21802 |
| Clone Number | S-R239 |
| Antibody Type | Recombinant mAb |
| Application | ICC, FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:500 | null |
| FCM | 1:500 | null |
Background
Fibroblast growth factor receptor 2 (FGFR2) is one of four FGFRs that encode a transmembrane receptor tyrosine kinase. FGFR2 signaling is associated with the proliferation, migration, and angiogenesis of carcinomas. FGFR2-overexpressed cancer might be a therapeutic target. Several FGFR2 inhibitors have been developed for the treatment of cancer patients with enhanced expression of FGFR2 signaling7FGFR2 has two isoforms, i.e., the IIIb type and the IIIc type based on the alternative splicing within the C-terminal half of the third Ig loop (D3) in the extracellular FGF binding domain, which are alternatively spliced by exon 8 and by exon 9, respectively. The spliced isoforms differ in binding ligands: FGFR2IIIb is a high-affinity receptor for FGF1, -3, -7, -10, and -22, whereas FGFR2IIIc binds FGF1, -2, -4, -6, -8, -9 -17 and-18. The FGFR2IIIb isoform is expressed mainly in epithelial cells, and it preferentially binds secreted FGF ligands from adjacent mesenchymal cells. In contrast, the FGFR2IIIc isoform is preferentially expressed in mesenchymal cells and usually binds ligands secreted from the adjacent epithelial cells.
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Picture
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Immunocytochemistry
ICC shows positive staining in KATO Ⅲ cells. Anti-FGFR2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
Negative control: ICC shows negative staining in SW620 cells. Anti-FGFR2 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (red).
