WB result of FABP4 Recombinant Rabbit mAb
Primary antibody: FABP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: undifferentiated 3T3-L1 whole cell lysate 20 µg
Lane 2: 3T3-L1 differentiated 4 days whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
Observed MW: 14 kDa
FABP4 Recombinant Rabbit mAb (S-1360-168)
FABP4 Recombinant Rabbit mAb (S-1360-168)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FABP4 |
| Synonyms | Fatty acid-binding protein, adipocyte; Adipocyte lipid-binding protein (ALBP); Adipocyte-type fatty acid-binding protein (A-FABP; AFABP); Fatty acid-binding protein 4 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P15090 |
| Clone Number | S-1360-168 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | 3T3-L1, rat heart |
| Predicted Reactivity | Pg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000-1:2000 | Hu |
| IF | 1:2000 | Hu |
Background
FABP4, also known as Adipocyte Fatty Acid Binding Protein (A-FABP) or aP2, is a small cytoplasmic protein belonging to the family of fatty acid-binding proteins (FABPs). It is widely expressed in various tissues, including adipose tissue, macrophages, small intestine, heart, and breast, with particularly high levels in adipocytes and macrophages. The primary function of FABP4 is to serve as an intracellular transporter of fatty acids. It facilitates the uptake, transport, and metabolism of fatty acids by binding to long-chain fatty acids (LCFAs) to form stable complexes. These complexes then shuttle the fatty acids to various intracellular compartments such as mitochondria, endoplasmic reticulum, or peroxisomes for processes like β-oxidation, esterification, or phospholipid synthesis. In addition to its role in fatty acid metabolism, FABP4 is also involved in regulating adipocyte differentiation and insulin sensitivity. Moreover, it plays a significant part in inflammatory responses and immune modulation.
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Western Blot
WB result of FABP4 Recombinant Rabbit mAb
Primary antibody: FABP4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 15 kDa
Observed MW: 14 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast. Anti-FABP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-FABP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-FABP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-FABP4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human breast. Anti-FABP4 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
