WB result of eNOS Recombinant Rabbit mAb
Primary antibody: eNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HUVEC whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 133 kDa
eNOS Recombinant Rabbit mAb (S-1885-94)
eNOS Recombinant Rabbit mAb (S-1885-94)
Price:
Regular price
$130.00 SGD
Regular price
Sale price
$130.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | eNOS |
| Synonyms | Nitric oxide synthase 3; Constitutive NOS (cNOS); EC-NOS; NOS type III (NOSIII); Nitric oxide synthase; endothelial (Endothelial NOS; eNOS); NOS3 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | P29474 |
| Clone Number | S-1885-94 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HUVEC, mouse liver, mouse spleen, rat liver |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
Endothelial nitric oxide synthase (eNOS), also known as nitric oxide synthase 3 (NOS3), is a critical enzyme primarily expressed in vascular endothelial cells, as well as in other tissues such as the myocardium, airway epithelium, and renal tubular cells. It catalyzes the conversion of L-arginine to L-citrulline, producing nitric oxide (NO) as a byproduct, which is a key signaling molecule for regulating vascular tone, promoting vasodilation, and maintaining cardiovascular homeostasis. eNOS activity is regulated by various stimuli, including shear stress, calcium influx, and phosphorylation at specific residues such as serine 1177. Dysfunction of eNOS, such as uncoupling due to oxidative stress or deficiency of cofactors like tetrahydrobiopterin (BH4), can lead to reduced NO production and contribute to endothelial dysfunction, a hallmark of cardiovascular diseases.
Picture
Picture
Western Blot
WB result of eNOS Recombinant Rabbit mAb
Primary antibody: eNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 133 kDa
WB result of eNOS Recombinant Rabbit mAb
Primary antibody: eNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 133 kDa
Observed MW: 133 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded mouse skeletal muscle (low expression). Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-eNOS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
