WB result of CENPB Recombinant Rabbit mAb
Primary antibody: CENPB Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 80 kDa
This blot was developed with high sensitivity substrate
CENPB Recombinant Rabbit mAb (S-1713-209)
CENPB Recombinant Rabbit mAb (S-1713-209)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CENPB |
| Synonyms | Major centromere autoantigen B; Centromere protein B (CENP-B) |
| Immunogen | Synthetic Peptide |
| Accession | P07199 |
| Clone Number | S-1713-209 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | A431 |
| Predicted Reactivity | Hm |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
CENPB protein, which stands for centromere protein B, is a crucial component of the centromere, a specialized region on chromosomes. It plays a vital role in ensuring proper chromosome segregation during cell division. CENPB is involved in the formation and maintenance of centromeric chromatin, helping to establish and stabilize the structure of the centromere. This protein also aids in the recruitment of other essential centromere-associated proteins, thereby facilitating the assembly of the kinetochore, a complex protein structure that forms on the centromere and is essential for the attachment of spindle microtubules during mitosis and meiosis. Mutations or abnormalities in CENPB can lead to chromosomal instability and are associated with various genetic disorders and cancers.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in A431 cells. Anti-CENPB antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
