WB result of CD28 Recombinant Rabbit mAb
Primary antibody: CD28 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: EL4.IL2 whole cell lysate 20 µg
Negative control: THP-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 12, 35~50 kDa
CD28 Recombinant Rabbit mAb (S-1815-10)
CD28 Recombinant Rabbit mAb (S-1815-10)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD28 |
| Synonyms | T-cell-specific surface glycoprotein CD28; TP44 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P10747 |
| Clone Number | S-1815-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | Jurkat, EL4.IL2 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| ICC | 1:50-1:500 | Hu, Ms |
Background
CD28 is a key costimulatory receptor predominantly expressed on T cells, which plays an essential role in T cell activation, proliferation, and survival. Upon binding to its ligands CD80 (B7-1) and CD86 (B7-2) expressed on antigen-presenting cells, CD28 provides a secondary signal that amplifies the initial T cell receptor engagement with the antigen. This costimulation is crucial for preventing T cell anergy and for the full execution of immune responses, including the production of cytokines and the development of memory T cells. CD28 also contributes to the induction and maintenance of regulatory T cells, and its signaling involves the activation of multiple downstream pathways, including the PI3K-AKT axis, which is important for cell metabolism and survival.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in Jurkat cells. Anti-CD28 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in EL4.IL-2 cells. Anti-CD28 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
