WB result of CBS Rabbit pAb
Primary antibody: CBS Rabbit pAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse kidney lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 61 kDa
CBS Rabbit Polyclonal Antibody
CBS Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CBS |
| Synonyms | Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P35520 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Ms, Rt |
| Positive Sample | mouse kidney, mouse liver, rat kidney, rat liver |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:250 | Ms, Rt |
| IF | 1:100-1:500 | Ms, Rt |
Background
Cystathionine beta-synthase (CBS) is a crucial enzyme in the trans-sulfuration pathway that catalyzes the conversion of homocysteine to cystathionine, which is subsequently converted to cysteine. It is a pyridoxal 5′-phosphate (PLP)-dependent enzyme with a modular three-domain structure. The N-terminal domain binds a heme cofactor that acts as a redox sensor, while the C-terminal domain contains a tandem CBS domain that binds S-adenosylmethionine (SAM), an allosteric activator. Mutations in the CBS gene can lead to homocystinuria, a condition associated with cardiovascular, ocular, skeletal, and central nervous system complications. CBS is also involved in hydrogen sulfide (H₂S) production, an important gasotransmitter with various physiological functions.
Picture
Picture
Western Blot
WB result of CBS Rabbit pAb
Primary antibody: CBS Rabbit pAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat liver lysate 20 µg
Lane 3: rat kidney lysate 20 µg
Negative control: rat skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 61 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-CBS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse skeletal muscle. Anti-CBS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CBS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-CBS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded rat skeletal muscle. Anti-CBS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse kidney. Anti-CBS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat kidney. Anti-CBS antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
