WB result of BST2 Recombinant Rabbit mAb
Primary antibody: BST2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Lane 4: mouse thymus lysate 20 µg
Negative control: C2C12 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 19 kDa
Observed MW: 28-35, 48-75 kDa
Product Details
Product Details
Product Specification
Host | Rabbit |
Antigen | BST2 |
Synonyms | Bone marrow stromal antigen 2; BST-2; HM1.24 antigen; CD317; Bst2 |
Immunogen | Recombinant Protein |
Location | Cytoplasm, Endosome, Cell membrane |
Accession | Q8R2Q8 |
Clone Number | S-2114-32 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | WB |
Reactivity | Ms |
Positive Sample | RAW264.7, mouse spleen, mouse thymus |
Purification | Protein A |
Concentration | 0.5 mg/ml |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
application | dilution | species |
WB | 1:1000 | Ms |
Background
BST2 protein, also known as bone marrow stromal antigen 2 or CD317, is a type of protein that plays significant roles in various biological processes. It is a type II transmembrane protein mainly expressed on the surface of cells such as fibroblasts, endothelial and cells some immune cells. One of its key functions is in the regulation of cell proliferation and differentiation. For example, in the bone marrow microenvironment, BST2 protein can influence the growth and development of hematopoietic cells by interacting with other molecules. Moreover, it is involved in the immune response. It can affect the activation and function of immune cells like T cells and B cells, thus participating in the regulation of the body's immune defense mechanism. Additionally, BST2 protein has been found to be associated with some diseases. Abnormal expression of BST2 protein may be related to the occurrence and development of certain cancers and autoimmune diseases. Therefore, research on BST2 protein is of great importance for understanding the mechanisms of cell biology and disease pathogenesis.
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Western Blot
