WB result of Bax Recombinant Rabbit mAb
Primary antibody: Bax Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22 kDa
Bax Recombinant Rabbit mAb (S-2603-124)
Bax Recombinant Rabbit mAb (S-2603-124)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Bax |
| Synonyms | Apoptosis regulator BAX |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Mitochondrion |
| Accession | Q07813 |
| Clone Number | S-2603-124 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | HEK-293, NIH/3T3, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Mk |
| IHC-P | 1:1000-1:5000 | Hu, Ms |
Background
Bax is a pro-apoptotic member of the Bcl-2 protein family that localizes to the cytosol of healthy cells but, upon activation by developmental death cues or intracellular stress such as DNA damage, undergoes conformational change and translocates to the outer mitochondrial membrane where it oligomerizes to form pores that permeabilize the membrane, thereby releasing cytochrome c and other apoptogenic factors into the cytosol to trigger the caspase cascade and ultimately apoptosis.
Picture
Picture
Western Blot
WB result of Bax Recombinant Rabbit mAb
Primary antibody: Bax Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22 kDa
WB result of Bax Recombinant Rabbit mAb
Primary antibody: Bax Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 22 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Bax antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Bax antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Bax antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
