Flow cytometric analysis of human peripheral blood cells labelling CD27 antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Amenian hamster IgG Isotype Control / (left panel). Goat Anti-Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ antibody at separately.
Armenian hamster Anti-Mouse/Rat/Human CD27 Antibody (S-R620)
Armenian hamster Anti-Mouse/Rat/Human CD27 Antibody (S-R620)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CD27 |
| Synonyms | Signal transducer CD24; Lymphocyte antigen 52 (Ly-52); M1/69-J11D heat stable antigen (HSA); Nectadrin; R13-Ag; X62 heat stable antigen; Cd24a; Ly-52 |
| Location | Cell membrane |
| Accession | P26842、P41272、Q501W2 |
| Clone Number | S-R620 |
| Antibody Type | Recombinant mAb |
| Application | FCM |
| Reactivity | Hu, Ms |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:200 | Hu, Ms, Rt |
Background
CD27 is a transmembrane protein that belongs to the tumor necrosis factor receptor superfamily (TNFRSF) and is primarily expressed on T cells, B cells, and natural killer (NK) cells. It activates NF-κB and JNK signaling pathways by binding to its ligand, CD70, thereby promoting the activation, proliferation, and differentiation of T cells. CD27 plays a crucial role in immune regulation, acting as an important co-stimulatory receptor for T and B cells, and is involved in modulating immune responses and the formation of memory cells. Additionally, CD27 is being studied as a potential target for anti-tumor immunotherapy.
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Flow cytometric analysis of C57BL/6 mouse splenocytes labelling CD27 antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Amenian hamster IgG Isotype Control / (left panel). Goat Anti-Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD19 - Brilliant Violet 421™ antibody at separately.
Flow cytometric analysis of SD Rat splenocytes labelling CD27 antibody at 1/200 dilution (1 μg) / (Red) compared with a Amenian hamster IgG (Black) Isotype Control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Amenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody.
