WB result of AMPKβ1/2 Recombinant Rabbit mAb
Primary antibody: AMPKβ1/2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Lane 5: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 30, 38 kDa
AMPKβ1/2 Recombinant Rabbit mAb (S-2728-26)
AMPKβ1/2 Recombinant Rabbit mAb (S-2728-26)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | AMPKβ1/2 |
| Synonyms | 5'-AMP-activated protein kinase subunit beta-1/2; AMPK subunit beta-1/2; AMPKb; AMPK |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q9Y478、O43741 |
| Clone Number | S-2728-26 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HepG2, HeLa, A549, 293T, HEK-293, NIH/3T3, mouse brain, mouse liver, rat brain, COS-7 |
| Predicted Reactivity | Bv, Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:200 | Hu, Ms |
| ICC | 1:100 | Hu, Ms |
Background
AMPKβ1/2 are indispensable scaffolding isoforms of the 5′-AMP-activated protein kinase (AMPK) complex that harbor a conserved carbohydrate-binding module (CBM) which docks glycogen and related oligosaccharides to allosterically sensitize the holoenzyme to cellular energy stress, while simultaneously anchoring the α-catalytic and γ-regulatory subunits through their C-terminal sequences to ensure correct heterotrimeric assembly, stable lysosomal or mitochondrial localization, and optimal phosphorylation-mediated activation by upstream kinases such as LKB1 and CaMKK2, thereby governing systemic metabolic fluxes including fatty-acid oxidation, glucose uptake, and autophagy in liver, skeletal muscle, and adipose tissue.
Picture
Picture
Western Blot
WB result of AMPKβ1/2 Recombinant Rabbit mAb
Primary antibody: AMPKβ1/2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 30, 38 kDa
WB result of AMPKβ1/2 Recombinant Rabbit mAb
Primary antibody: AMPKβ1/2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 30, 38 kDa
WB result of AMPKβ1/2 Recombinant Rabbit mAb
Primary antibody: AMPKβ1/2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 30, 38 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-AMPKβ1/2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-AMPKβ1/2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-AMPKβ1/2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-AMPKβ1/2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- AMPKβ1/2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti- AMPKβ1/2 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
