Mycoplasma Detection Kit (qPCR)
Mycoplasma Detection Kit (qPCR)
Price:
Regular price
$968.25 USD
Regular price
Sale price
$968.25 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Usage |
1. Preparation:
【Note】:If it is added during extractionIC, then prepareqPCR MIXWhen equal volume DNAdiluent instead IC.
（3) qPCRAdd sample: ①Place the required reagents on ice, shake gently to mix, centrifuge briefly, and add the sample (total volume 25 μL): ②The experiment can use sterile enzyme-free eight-tube or 96When performing the reaction in the well plate, it is necessary to remove the bubbles in the reaction system and centrifuge to the bottom of the tube to prepare for the reaction. （4) qPCRReaction program parameter settings: The United States ABI 7500 Real-Time PCR System, software version 2.06For example. A,exist Experimental PropertiesCreate a blank new program on the page as shown below:  ①Experiment name: Modify the experiment name.②Experiment type: Select a program (Quantitation-Standard Curve).③Reagents: Select the experimental method (TaqMan® Reagents). B, create the product detection probe, selectselect  ①interface, click ②Create a detection probe and an internal reference probe. TargetNamemiddleEdit the probe name and name it.Reporter③andQuencher④Select the desired fluorophore and quencherThis product detects fluorescent groups.FAM, the quenching group isNone, selection of internal reference fluorescent groupsCY5, the quenching group isNone, the reference fluorescence isROX.pointhit ⑤Create samples, in the corresponding Sample name⑥Name the sample in the column. C,existPlate Setup  ①Interface, place the positive control wells at Task②onecolumnset upPlacefor “S”③, the no-template control NTCKong ZaiTask②One column is set to“N”④, place the sample hole to be tested inTask②One column is set to “U”⑤, and in the corresponding Sample nameName the sample in a column (Figure LegendonlyforGinsengTest). D, set up the reaction program:  【Note】Some devices do not allow the fluorescence collection step to be set to 30sor shorter; use ABI7000、 ABI 7300and ABI 7500At least 31sFor other equipment, please consult the instrument manufacturer.  E, click RunInterface "Start Run"Button to proceedPCRDetermination. 3. Result Analysis The United States ABI 7500 Real-Time PCR System, software version 2.06For example. 1,exist Analysisof Amplification Plot①In the panel, clickReanalyse②, initially check whether the shape of the amplification curve is normal. AnalysisofViewWell Table③In the panel, CtOne column can read the sample to be tested CtDetection value. (The illustration is for reference only)  2The parameter settings for result analysis need to be based on the specific model and software version used, and can generally be automatically interpreted by the instrument. 3、NTC,Positive PCThe results must meet 
|
||||||||||||||||||||||||
| Description | Mycoplasmas are prokaryotes that commonly contaminate cells in the laboratory. The main sources of contamination include cells and virus strains, as well as animal-derived raw materials such as serum, chicken embryos, and primary cells, and the operators themselves. According to statistics, approximately 15%-35% of cells are contaminated with mycoplasmas. Mycoplasmas can alter a range of host cell characteristics, including structure and function, leading to inaccurate experimental results. They can also interfere with cell metabolism and growth, alter nucleic acid synthesis, affect cell antigenicity, cause chromosomal changes, interfere with viral replication, and mimic viral functions. This test kit offers high specificity and sensitivity, and can be used to qualitatively detect mycoplasma contamination in master cell banks, working cell banks, viral seed batches, control cells, and cells used in clinical treatment. It can be used in conjunction with the Host Cell Residual DNA (Magnetic Bead Method) Sample Pretreatment Kit (abs60544). Detection Principle: According to <2.6.7> of the European Pharmacopoeia, nucleic acid amplification (NAT) can replace culture and indicator cell culture methods after appropriate validation. Fluorescent probe (TaqMan®) qPCR is characterized by high sensitivity and specificity, making TaqMan® the preferred method for mycoplasma detection. TaqMan® fluorescent probes are oligonucleotide probes with a fluorescent group (reporter) at the 5' end, such as FAM, CY5, or VIC, and a quencher at the 3' end, such as TAMRA or BHQ1. During PCR amplification, a specific fluorescent probe is added along with a pair of primers. When the probe is intact, the fluorescent signal emitted by the fluorescent group is absorbed by the quencher. During PCR amplification, the 5'-3' exonuclease activity of the Taq enzyme cleaves and degrades the probe, separating the fluorophore and quencher. This allows the fluorescence monitoring system (fluorescence quantitative PCR instrument) to detect a fluorescent signal. This means that each time a DNA strand is amplified, a fluorescent molecule is formed, ensuring that the accumulation of the fluorescent signal is completely synchronized with the formation of PCR products. This kit utilizes a multiplex PCR approach, using two different fluorescent probes to detect the target sequence and the internal reference, respectively. Product components:
|
