IF shows positive staining in paraffin-embedded human tonsil (case 1). Anti-PD-1 (Alexa Fluor® 555 Conjugate) antibody was used at 1/500 dilution (Red) and incubated 45min at RT. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
PD-1 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-035-25)
PD-1 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-035-25)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PD-1 |
| Synonyms | CD279, PDCD1 |
| Immunogen | Synthetic Peptide |
| Location | Membrane |
| Accession | Q15116 |
| Clone Number | SDT-035-25 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 555 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:200 | Hu |
Background
Programmed cell death protein 1, also known as PD-1 and CD279 (cluster of differentiation 279), is a protein on the surface of T and B cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. This prevents autoimmune diseases, but it can also prevent the immune system from killing cancer cells. PD-1 is an immune checkpoint and guards against autoimmunity through two mechanisms. First, it promotes apoptosis (programmed cell death) of antigen-specific T-cells in lymph nodes. Second, it reduces apoptosis in regulatory T cells (anti-inflammatory, suppressive T cells).
Picture
Picture
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil (case 2). Anti-PD-1 (Alexa Fluor® 555 Conjugate) antibody was used at 1/500 dilution (Red) and incubated 45min at RT. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
