PCNA Recombinant Rabbit mAb (PE Conjugate) (SDT-297-116)

Catalog Number: S0B1761 Application: FCM Reactivity: Human,Mouse,Rat Conjugation: PE Brand: Starter

Price:

$180 USD

Size: 25μl 100μl

Quantity:

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Product Details

Product Specification

Host	Rabbit
Antigen	PCNA
Synonyms	Proliferating cell nuclear antigen; Cyclin
Immunogen	Synthetic Peptide
Location	Nucleus
Accession	P12004
Clone Number	SDT-297-116
Antibody Type	Recombinant mAb
Isotype	IgG
Application	ICFCM
Reactivity	Hu, Ms, Rt
Predicted Reactivity	Av, Bv, Hm
Purification	Protein A
Concentration	1 mg/ml
Conjugation	PE
Physical Appearance	Liquid
Storage Buffer	PBS, 1% BSA, 0.3% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

Dilution

application	dilution	species
ICFCM	1:800	Hu

Background

Proliferating cell nuclear antigen (PCNA) is a type of DNA clamp that acts as a DNA polymerase in eukaryotic cells δ. The processing factor of is crucial for replication. PCNA is a homologous trimer that achieves its processability by surrounding DNA, where it serves as a scaffold for recruiting proteins involved in DNA replication, DNA repair, chromatin remodeling, and epigenetics. It is expressed in most tumors as a proliferative antigen.

Picture

FC

Flow cytometric analysis of PCNA expression on 4% PFA fixed 90% methanol permeabilized HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cell) was stained with Phycoerythrin Rabbit IgG Isotype Control (Black line histogram) and SDT PCNA Recombinant Rabbit mAb (PE Conjugate) (Red line histogram) at 1/800 dilution (0.25 μg), cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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PCNA Recombinant Rabbit mAb (PE Conjugate) (SDT-297-116)