IF shows positive staining in paraffin-embedded human thyroid cancer. Anti-PAX8 Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
PAX8 Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-R509)
PAX8 Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-R509)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PAX8 |
| Synonyms | Paired box protein Pax-8 |
| Location | Nucleus |
| Accession | Q06710 |
| Clone Number | SDT-R509 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Positive Sample | Human thyroid cancer |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:200 | Hu |
Background
Paired box gene 8, also known as PAX8, is a protein which in humans is encoded by the PAX8 gene. This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins which contain a paired box domain, an octapeptide, and a paired-type homeodomain. The PAX gene family has an important role in the formation of tissues and organs during embryonic development and maintaining the normal function of some cells after birth. PAX8 (and PAX2) is one of the important regulators of urogenital system morphogenesis.
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Picture
Immunofluorescence
