IF shows positive staining in paraffin-embedded human bladder cancer. Anti-p27 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
p27 Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (SDT-361-310)
p27 Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (SDT-361-310)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | p27 |
| Synonyms | Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Cdkn1b; KIP-1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P46414 |
| Clone Number | SDT-361-310 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Fg, Ct |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:50 | Hu |
Background
p27 KIP1 is a member of the Cyclin Inhibitory Protein/Kinase Inhibitory Protein (CIP/KIP) family of cyclin-dependent kinase (Cdk) inhibitors. It plays roles in all phases of the cell division cycle. Its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes enforces the G1 restriction point. Thus, important cellular functions as the cell division cycle, respiration, RNA processing, translation, and cell adhesion, are under p27 KIP1 regulation. Loss or reduced expression is associated with poor outcome in carcinoma of breast, prostate, GI tract and lung.
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Immunofluorescence
IF shows positive staining in paraffin-embedded human prostatic cancer. Anti-p27 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
